Using 5-(4-aminoantipyrineazo)-8-hyrroxyquinoline as Complexometry Reagent with Cupper (II) and Zinc (II) Ions and its Biological Activity

  The complexes of azo dye 5-(4-aminoantipyrineazo)-8-hyroxyquinoline (L) with cupper (II) and zinc (II) Ions were synthesized. Characterization of these azo dyes complexes have been done on the basis of elemental analysis and IR techniques. The work involves a study of optimum conditions (time, pH, sequence of addition and temperature effects) for forming the complexes. The spectra of the complexes have been studied for a range of concentrations which Lambert – Beer's law were obeyed with sensitivity of the spectrophotometric method in terms of molar absorptivity 1.6×104  and 1.2×104  l .mol-1.cm-1 for zinc and cupper azo dyes complexes respectively .The stoichiometry of the complexes has been found to be 1:2 (metal : ligand). The overall stability constants were determined by the corresponding solutions method. The interferences effects of the foreign ions were studied. The biological activity of two complexes with two type of Bacteria (Clostridium botulinum and Escherichia Coli) were also studied

Modeling and control of quartz crystal operated in liquid for bio-sensing application

Nowadays the applications of sensors are a very promising research field since they find application in many different areas. Biosensors are an increasingly important technology in the detection of compounds ranging from pesticides to biological weapons. Typically, biosensors consist of a biological macromolecule that is immobilized on the surface of a signal transducer. As the macromolecule binds specifically to the ligand being detected, the signal transducer can measure a physical change due to the binding event. One promising type of detector is the Quartz Crystal Microbalance (QCM). The QCM is a piezoelectric mass-sensing device. A QCM device works by sending an electrical signal through a gold-plated quartz crystal, which causes a vibration at some resonant frequency. The QCM then measures the frequency of oscillation in the crystal. When used as a biosensor, the QCM can detect changes in frequency of the crystal due to changes in mass on the surface of the crystal. In the present work, all related equations and data base related to biosensors piezoelectric devices will be considered in the developed model. The model will describe biosensors limitations and capacities together with measurement resolutions and errors. The work will includes build of software program using Microsoft visual studio C# 2010.net frame work 4.0 and all available theoretical and empirical formulas and program data base will include the data for QCM sensors and resonance frequencies, this program will include all cases needed to be studied for the present work (bio sensing). Many sensors can be used for QCM, the present work will be limited to TSM sensors, and the proposed software for Bio-sensing. The frequency signal is detected by a frequency counter and processed electronically in a separate computer. The QCM sensor used is 19.5 MHZ using buffer media in the testing procedure. The result according to z-methods indicates that while the resonance frequencies increase the small amount of despite material can be easily detected. With the data extracted for (5 MHZ) sensors with effective area of 14 mm2 , the results show that as the change in resonance frequency increase as the despite material increase. The film thickness of deposit material increase as the natural frequencies of sensors decreased

A genetic algorithm for shortest path with real constraints in computer networks

The shortest path problem has many different versions. In this manuscript, we proposed a muti-constrained optimization method to find the shortest path in a computer network. In general, a genetic algorithm is one of the common heuristic algorithms. In this paper, we employed the genetic algorithm to find the solution of the shortest path multi-constrained problem. The proposed algorithm finds the best route for network packets with minimum total cost, delay, and hop count constrained with limited bandwidth. The new algorithm was implemented on four different capacity networks with random network parameters, the results showed that the shortest path under constraints can be found in a reasonable time. The experimental results showed that the algorithm always found the shortest path with minimal constraints

Spread, circulation, and evolution of the Middle East respiratory syndrome coronavirus

The Middle East respiratory syndrome coronavirus (MERS-CoV) was first documented in the Kingdom of Saudi Arabia (KSA) in 2012 and, to date, has been identified in 180 cases with 43% mortality. In this study, we have determined the MERS-CoV evolutionary rate, documented genetic variants of the virus and their distribution throughout the Arabian peninsula, and identified the genome positions under positive selection, important features for monitoring adaptation of MERS-CoV to human transmission and for identifying the source of infections. Respiratory samples from confirmed KSA MERS cases from May to September 2013 were subjected to whole-genome deep sequencing, and 32 complete or partial sequences (20 were ≥99% complete, 7 were 50 to 94% complete, and 5 were 27 to 50% complete) were obtained, bringing the total available MERS-CoV genomic sequences to 65. An evolutionary rate of 1.12 × 10−3 substitutions per site per year (95% credible interval [95% CI], 8.76 × 10−4; 1.37 × 10−3) was estimated, bringing the time to most recent common ancestor to March 2012 (95% CI, December 2011; June 2012). Only one MERS-CoV codon, spike 1020, located in a domain required for cell entry, is under strong positive selection. Four KSA MERS-CoV phylogenetic clades were found, with 3 clades apparently no longer contributing to current cases. The size of the population infected with MERS-CoV showed a gradual increase to June 2013, followed by a decline, possibly due to increased surveillance and infection control measures combined with a basic reproduction number (R0) for the virus that is less than 1

Adeno-Associated Viral Transfer of Glyoxalase-1 Blunts Carbonyl and Oxidative Stresses in Hearts of Type 1 Diabetic Rats

Accumulation of methylglyoxal (MG) arising from downregulation of its primary degrading enzyme glyoxalase-1 (Glo1) is an underlying cause of diabetic cardiomyopathy (DC). This study investigated if expressing Glo1 in rat hearts shortly after the onset of Type 1 diabetes mellitus (T1DM) would blunt the development of DC employing the streptozotocin-induced T1DM rat model, an adeno-associated virus containing Glo1 driven by the endothelin-1 promoter (AAV2/9-Endo-Glo1), echocardiography, video edge, confocal imaging, and biochemical/histopathological assays. After eight weeks of T1DM, rats developed DC characterized by decreased E:A ratio, fractional shortening, and ejection fraction, and increased isovolumetric relaxation time, E: e’ ratio, and circumferential and longitudinal strains. Evoked Ca2+ transients and contractile kinetics were also impaired in ventricular myocytes. Hearts from eight weeks T1DM rats had lower Glo1 and GSH levels, elevated carbonyl/oxidative stress, microvascular leakage, inflammation, and fibrosis. A single injection of AAV2/9 Endo-Glo1 (1.7×1012 viron particles/kg) one week after onset of T1DM, potentiated GSH, and blunted MG accumulation, carbonyl/oxidative stress, microvascular leakage, inflammation, fibrosis and impairments in cardiac and myocyte functions that develop after eight weeks of T1DM. These new data indicate that preventing Glo1 downregulation by administering AAV2/9-Endo-Glo1 to rats one week after the onset of T1DM, blunted the DC that develops after eight weeks of diabetes by attenuating carbonyl/oxidative stresses, microvascular leakage, inflammation, and fibrosis

Elemental Study of Bangladeshi Fish Samples Using PIGE Technique

Proton induced gamma emission (PIGE) method has been developed at the Accelerator Laboratory of Atomic Energy Center, Dhaka (AECD) for the investigation of various human health related specimens. In the present study PIGE analytical technique has been used for the determination of numerous trace elements in some commonly used fish samples which were collected from Islampur area at Savar. During the experimental work the γrays emitted from the prepared fish samples were measured using a Highpurity Germenium (HPGe) detector. The detected 18 elements were Iron, Calcium, Sodium, Magnesium, Aluminum, Potassium, Vanadium, Chromium, Manganese, Copper, Zinc, Selenium, Bromine, Rubidium, Strontium, Cadmium, Iodine, Lead etc. The objective of this study was to measure the concentrations of these trace elements in the collected fish samples and to investigate whether their concentrations are harmful for human health by comparison with limits set by WHO/FAO. The results obtained indicate that Fe, K and Ca concentrations were relatively high in all samples as compared with the other elements. The measured elemental concentration of Fe, Mn, Zn, Cu, Cr and V in all fish samples were found to be above the WHO/FAO recommended limits

Prospects of Hybrid Energy in Saudi Arabia, Exploring Irrigation Application in Shaqra

Dynamics in rainfall patterns due to climate change are posing a threat to crop production globally. The core issue of food security is expected to intensify, and improving crop yield using motorized power irrigation mechanisms can help in curtailing the impact of drought and changing weather patterns to meet the crop water requirement. To meet the energy demand of irrigation systems, this paper explores the use of hybrid energy sources, i.e., wind and solar energy, taking Shaqra Saudi Arabia as case study. This paper presents a systematic case study that evaluates crop water requirements for 3 different crops using the United Nations Food and Agriculture Organization’s software CROPWAT 8.0 and converts the water requirement into energy demand to design the water pumping system. The energy requirement water pumping system is used to design a hybrid energy system using HOMER PRO 3.14.4 that can reliably meet the energy demand. The results suggests that, contrary to the common consideration in Saudi Arabia, a hybrid of wind and solar energy proves to be more cost effective and yields a higher amount of energy. The results suggest that a significant reduction in cost can be achieved with a hybrid energy system as compared to a solar PV system only

Surgical Approaches to Congenital Anomalies of Esophagus

With prevalence of about 1 in 3000 live births, pediatric surgeons commonly deal with esophageal abnormalities, which may provide substantial clinical complications. Surprisingly, the embryologic processes underlying esophageal atresia (EA) with or without tracheoesophageal fistula (TEF), one of the hallmark disease entities of pediatric surgery, have only lately been largely uncovered. When it comes to the treatment of congenital esophageal abnormalities, notably esophageal atresia and tracheoesophageal fistula, surgical methods are essential. In order to address the anatomical abnormalities and restore normal function, surgical correction is often necessary in the care of congenital esophageal anomalies, including esophageal atresia and tracheoesophageal fistula. In this review we are going to cover surgical approaches to repair those malformations, long-term outcomes, and latest developments in esophageal surgical approaches

Risk Assessment Of In-Vitro Fertilization, Review Article

Since its remarkable inception in 1978, IVF has garnered significant public interest.   Currently, assisted reproductive technology is widely accessible in most developed countries, and the methods employed have significantly evolved since its inception.   Advancements in laboratory technology and clinical practice have enabled IVF to develop into a medical process that is highly efficient, safe, easily accessible, and comparatively affordable. Over 2 million children conceived by IVF have been born so far, and it is probable that ongoing improvements will increase its attractiveness and suitability. There has been a rising interest in the topic of risk assessment in IVF in recent years, with a significant amount of research focused on detecting and reducing the potential dangers linked to the operation.   This review article seeks to offer a thorough and all-encompassing analysis of the present understanding of risk assessments in IVF, encompassing the diverse range of risks and complications linked to the operation

Treatment of Disseminated Mycobacterial Infection with High-Dose IFN-γ in a Patient with IL-12Rβ1 Deficiency

IFN-γ has been used in the treatment of IL-12Rβ1 deficiency patients with disseminated BCG infection (BCGosis), but the optimal dose to reach efficacy is not clear. We used IFN-γ in the treatment of a 2.7-year-old patient with IL-12Rβ1 deficiency and refractory BCG-osis. IFNγ was started at a dose of 50 μg/m2 3 times per week. The dose was upgraded to 100 mcg/m2 after 3 months, then to 200 mcg/m2 6 months afterwards. Serum mycobactericidal activity and lymphocytes number and function were evaluated throughout the study. There was no clinical response to IFN-γ with 50 or 100 μg/m2 doses. However, there was some response to the 200 μg/m2 dose with no additional adverse effects. The serum mycobactericidal activity was not significantly different during the whole treatment period. Lymphocytes proliferation in response to PHA was significantly higher after 3 months of using the highest dose as compared to the lowest dose. The tuberculin skin test reaction remained persistently negative. We conclude that in a patient with IL-12Rβ1 deficiency, IFN-γ at a dose of 200 μg/m2, but not at lower dosages, was found to have a noticeable clinical effect with no additional adverse effects